N-GeneS

Goals:

The massively parallel sequencing technology known as next-generation sequencing (NGS) has revolutionized the biological sciences. With its ultra-high throughput, scalability, and speed, NGS enables researchers to perform a wide variety of applications and study biological systems at a level never before possible. These courses are designed for scientists and clinicians with little or no experience in exome/DNA sequencing. This aims to provide the experimental and bioinformatics skills required to prepare samples, undertake exome sequencing and the subsequent identification of single nucleotide variations (SNVs) and short Insertions/Deletions (Indels). In addition, it will provide information on the interpretation of the sequencing data including disease association.

There are six sessions of Next Generation Sequencing courses. Session I, II, III are general principles for NGS technical basis and bioinformatic analysis of data. Session IV is about the application of NGS in clinical practise. Session V is practical for everyone who want to try to interpret variants and analyse data. Session VI presents real cases from our experience through NGS.

Sessions:

I) Next Generation Sequencing-Introduction

II) Technical basis of Next Generation Sequencing (NGS)

III) Bioinformatics analysis of NGS data

IV) Clinical Next Generation Sequencing

V) Cases to solve - Workshop

VI) Case reports

Each session include: Main course (general info, presentation, script), Quiz, Chat/Forum

How to use the platform N-GeneS
Are you interested in Next Generation Sequencing technology? On this platform you can find all information you need to understand the technical basis, the bioinformatic tools/algorithms for data analysis and clinical application.
You can begin from session I that includes a short introduction, the history of sequencing and the NGS glossary. Then in session II and session III to understand the principles of NGS technology and how it works. In session III you will learn about the bioinformatic analysis steps and processing the NGS data. To continue, in session IV you will learn about the clinical NGS and how to interpret variants using specific tools and guidelines. In each of these session you can check your knowledge answering the questions.
In session V, there are one workshop, in which have been annotated NGS datasets from different cases. Download the VarAFT application, read the manual and protocol, download the data and do the analysis on your own. After the analysis, write your report and attach it to the "Case#-Assignment". In session VI, you can find case reports from our experience.
In each session there is chat and forum module if you have questions.

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